Neuronal human BACE1 knock-in induces systemic diabetes in mice

Acknowledgements The authors thank S. Tammireddy (Diabetes and Cardiovascular Science, University of the Highlands and Islands, Inverness, UK) for technical support with the lipidomics component. Funding We would like to thank R. Simcox, Romex Oilfield Chemicals, for financial support for KP, and acknowledge additional contributions from the Scottish Alzheimer’s Research UK network for the lipidomics work. The College of Life Science and Medicine, University of Aberdeen, sponsored the imaging study. MD was funded by British Heart Foundation and Diabetes UK; NM was funded by a British Heart Foundation Intermediate Fellowship; KS was funded by a European Foundation for the Study of Diabetes/Lilly programme grant; and RD was funded by an Institute of Medical Sciences PhD studentship.Peer reviewedPublisher PDFPublisher PD

Biomarker Discovery in Animal Health and Disease: The Application of Post-Genomic Technologies

The causes of many important diseases in animals are complex and multifactorial, which present unique challenges. Biomarkers indicate the presence or extent of a biological process, which is directly linked to the clinical manifestations and outcome of a particular disease. Identifying biomarkers or biomarker profiles will be an important step towards disease characterization and management of disease in animals. The emergence of post-genomic technologies has led to the development of strategies aimed at identifying specific and sensitive biomarkers from the thousands of molecules present in a tissue or biological fluid. This review will summarize the current developments in biomarker discovery and will focus on the role of transcriptomics, proteomics and metabolomics in biomarker discovery for animal health and disease

Winter distribution of willow flycatcher subspecies

The article presents a study which examines the migratory connectivity in the Willow Flycatcher subspecies throughout the winter range using mitochondrial DNA sequences as well as the morphological characteristics of 68 museum specimens obtained in the winter range in Costa Rica. It notes that the findings reveal that the overlapping and distinct regions of the winter range were occupied by the four subspecies. The specific breeding of these species is connected to the winter grounds

An audio-visual educational program adapted to the specific needs of the seventh, eighth, and ninth grades of the High Point Junior High School

The increasing interest in the improvement of instruction in the public schools of North Carolina for the past few years focused attention on the audio-visual education program now being developed in this country. Many indictments have been hurled against the effectiveness of our instruction in language arts and other areas of the curriculum by business men, members of college faculties, and more recently by examiners of military candidates. They realize the necessity for knowing and using a wide variety of materials in securing the development of functional knowledge and understanding. As a result, teachers are realizing that audio-visual aids give to pupils insight, understanding and the ability to remember

Online Skills-Based Motivational Interviewing Lab: Tools to Prepare Students for Field Education

These are the slides from a conference session at the 2021 Social Work Distance Education Conference. This interactive workshop focuses on a skills-based Motivational Interviewing (MI) Lab piloted with first year distance education MSW students. The MI Lab equips students with a humanistic approach encompassing engagement skills appropriate across diverse populations and field settings. During this workshop, participants will learn the lab design, outcomes, and students’ feedback on how this skills-based lab improved their confidence and performance in field education. To learn more about this lab: https://socialwork.columbia.edu/news/new-lab-grounds-first-year-msw-students-in-motivational-interviewing/

Management of the Brown-Headed Cowbird: Implications for Endangered Species and Agricultural Damage Mitigation

The brown-headed cowbird (Molothrus ater; cowbird) is unique among North American blackbirds (Icteridae) because it is managed to mitigate the negative effects on endangered songbirds and economic losses in agricultural crops. Cowbird brood parasitism can further affect species that are considered threatened or endangered due to anthropogenic land uses. Historically, cowbirds have often been culled without addressing ultimate causes of songbird population declines. Similar to other North American blackbirds, cowbirds depredate agricultural crops, albeit at a lower rate reported for other blackbird species. Conflicting information exists on the extent of agricultural damage caused by cowbirds and the effectiveness of mitigation measures for application to management. In this paper, we reviewed the progress that has been made in cowbird management from approximately 2005 to 2020 in relation to endangered species. We also reviewed losses to the rice (Oryza sativa) crop attributed to cowbirds and the programs designed to reduce depredation. Of the 4 songbird species in which cowbirds have been managed, both the Kirtland’s warbler (Dendroica kirtlandii) and black-capped vireo (Vireo atricapilla) have been removed from the endangered species list following population increases in response to habitat expansion. Cowbird trapping has ceased for Kirtland’s warbler but continues for the vireo. In contrast, least Bell’s vireo (V. bellii pusillus) and southwestern willow flycatcher (Empidonax traillii extimus) still require cowbird control after modest increases in suitable habitat. Our review of rice depredation by cowbirds revealed models that have been created to determine the number of cowbirds that can be taken to decrease rice loss have been useful but require refinement with new data that incorporate cowbird population changes in the rice growing region, dietary preference studies, and current information on population sex ratios and female cowbird egg laying. Once this information has been gathered, bioenergetic and economic models would increase our understanding of the damage caused by cowbirds

Decellularised extracellular matrix-derived peptides from neural retina and retinal pigment epithelium enhance the expression of synaptic markers and light responsiveness of human pluripotent stem cell derived retinal organoids

Tissue specific extracellular matrices (ECM) provide structural support and enable access to molecular signals and metabolites, which are essential for directing stem cell renewal and differentiation. To mimic this phenomenon in vitro, tissue decellularisation approaches have been developed, resulting in the generation of natural ECM scaffolds that have comparable physical and biochemical properties of the natural tissues and are currently gaining traction in tissue engineering and regenerative therapies due to the ease of standardised production, and constant availability. In this manuscript we report the successful generation of decellularised ECM-derived peptides from neural retina (decel NR) and retinal pigment epithelium (decel RPE), and their impact on differentiation of human pluripotent stem cells (hPSCs) to retinal organoids. We show that culture media supplementation with decel RPE and RPE-conditioned media (CM RPE) significantly increases the generation of rod photoreceptors, whilst addition of decel NR and decel RPE significantly enhances ribbon synapse marker expression and the light responsiveness of retinal organoids. Photoreceptor maturation, formation of correct synapses between retinal cells and recording of robust light responses from hPSC-derived retinal organoids remain unresolved challenges for the field of regenerative medicine. Enhanced rod photoreceptor differentiation, synaptogenesis and light response in response to addition of decellularised matrices from RPE and neural retina as shown herein provide a novel and substantial advance in generation of retinal organoids for drug screening, tissue engineering and regenerative medicine